Early Clinical Insights into Human Milk Analysis


The FDA has recently approved human milk analyzers for use in healthcare settings. While this is very exciting, it also brings up a lot of questions. When should we sample? How do we minimize interference with breastfeeding? What do these results mean? With words like “high and low” and “good and bad” circulating in healthcare, we need to be especially careful with our messaging surrounding human milk analysis results. Let’s take a deep dive into understanding the normal physiologic ranges of human milk in terms of energy, fat, protein, and carbohydrate. We will also look at case studies and discuss how milk analysis can impact nutrition interventions in the health care setting.

Learning Objectives

Define the typical physiologic variance ranges of energy content and the three macronutrients in human milk

Detail methods of human milk analysis sampling and individualized fortification

Describe two clinical applications of human milk analysis and changes to interventions in high-risk infants

Performance Indicators

2.3 Collaborates with inter- and intra-professional team members to achieve common goals and to optimize delivery of services

4.1 Demonstrates sound professional judgment and strategic thinking in practice

7.4 Participates in and leads quality control and improvement activities to improve delivery of services


Laura Serke

NICU Dietitian

University of Louisville Hospital

Stephanie Merlino Barr

NICU Dietitian

MetroHealth Medical Center


Anna Busenburg

Neonatal Dietitian

Cincinnati Children's Hospital Medical Center

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